Open letter from The BMJ to Mark Zuckerberg
— Read on www.bmj.com/content/375/bmj.n2635/rr-80
The epidemiological relevance of the COVID-19-vaccinated population is increasing – The Lancet Regional Health – Europe
— Read on www.thelancet.com/journals/lanepe/article/PIIS2666-7762(21)00258-1/fulltext
Abstract 10712: Mrna COVID Vaccines Dramatically Increase Endothelial Inflammatory Markers and ACS Risk as Measured by the PULS Cardiac Test: a Warning | Circulation
— Read on www.ahajournals.org/doi/10.1161/circ.144.suppl_1.10712
Increases in COVID-19 are unrelated to levels of vaccination across 68 countries and 2947 counties in the United States
— Read on www.ncbi.nlm.nih.gov/pmc/articles/PMC8481107/
Large-scale COVID-19 vaccinations are currently underway in many countries in response to the COVID-19 pandemic. Here, we report, besides generation of neutralizing antibodies, consistent alterations in hemoglobin A1c, serum sodium and potassium levels, coagulation profiles, and renal functions in healthy volunteers after vaccination with an inactivated SARS-CoV-2 vaccine. Similar changes had also been reported in COVID-19 patients, suggesting that vaccination mimicked an infection. Single-cell mRNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) before and 28 days after the first inoculation also revealed consistent alterations in gene expression of many different immune cell types. Reduction of CD8+ T cells and increase in classic monocyte contents were exemplary. Moreover, scRNA-seq revealed increased NF-κB signaling and reduced type I interferon responses, which were confirmed by biological assays and also had been reported to occur after SARS-CoV-2 infection with aggravating symptoms. Altogether, our study recommends additional caution when vaccinating people with pre-existing clinical conditions, including diabetes, electrolyte imbalances, renal dysfunction, and coagulation disorders.
— Read on www.nature.com/articles/s41421-021-00329-3
Colorectal cancer (CRC) is the third most common cancer worldwide and still lacks effective therapy. Ivermectin, an antiparasitic drug, has been shown to possess anti-inflammation, anti-virus, and antitumor properties. However, whether ivermectin affects CRC is still unclear. The objective of this study was to evaluate the influence of ivermectin on CRC using CRC cell lines SW480 and SW1116. We used CCK-8 assay to determine the cell viability, used an optical microscope to measure cell morphology, Annexin-V/7-AAD kit to determine cell apoptosis, used Caspase 3/7 Activity Apoptosis Assay Kit to evaluate Caspase 3/7 activity, used Western blot to determine apoptosis-associated protein expression, and used flow cytometry and fluorescence microscope to determine the reactive oxygen species (ROS) levels and cell cycle. The results demonstrated that ivermectin dose-dependently inhibited colorectal cancer SW480 and SW1116 cell growth, followed by promoting cell apoptosis and increasing Caspase-3/7 activity. Besides, ivermectin upregulated the expression of proapoptotic proteins Bax and cleaved PARP and downregulated antiapoptotic protein Bcl-2. Mechanism analysis showed that ivermectin promoted both total and mitochondrial ROS production in a dose-dependent manner, which could be eliminated by administering N-acetyl-l-cysteine (NAC) in CRC cells. Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed. Finally, ivermectin at low doses (2.5 and 5 µM) induced CRC cell arrest. Overall, ivermectin suppressed cell proliferation by promoting ROS-mediated mitochondrial apoptosis pathway and inducing S phase arrest in CRC cells, suggesting that ivermectin might be a new potential anticancer drug therapy for human colorectal cancer and other cancers.
— Read on www.frontiersin.org/articles/10.3389/fphar.2021.717529/full
SARS-CoV-2 Spike Protein Elicits Cell Signaling in Human Host Cells: Implications for Possible Consequences of COVID-19 Vaccines
— Read on www.ncbi.nlm.nih.gov/pmc/articles/PMC7827936/
Adenoviral Vector DNA- and SARS-CoV-2 mRNA-Based Covid-19 Vaccines: Possible Integration into the Human Genome – Are Adenoviral Genes Expressed in Vector-based Vaccines? – ScienceDirect
— Read on www.sciencedirect.com/science/article/pii/S0168170221001738
Twenty-year genetic trail behind Covid’s creation
— Read on www.conservativewoman.co.uk/twenty-year-genetic-trail-behind-covids-creation/
If you enjoy naturally acquired immunity, there isn’t even any need to care about your Ab titer at all. You may get some mild symptoms if you happen to get infected while your Ab titer is low but, as soon as your immune memory gets the wake-up call, it’ll provide you with very potent antibodies that can deal more effectively with all of the variants – even at relatively low concentration – than vaccinal anti-S Abs do.
— Read on www.geertvandenbossche.org/post/repetitio-est-mater-studiorum